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Abstract
The zona-free hamster ovum was evaluated as a substitute for human o va in the assessment of the fertilizing capacity of human spermatozoa. Zona-intact ova completely resisted sperm penetration. Using nonpreincu bated spermatozoa sperm penetration of zona-free ova began 4-5 hours after insemination. However when spermatozoa were preincubated in a modified Krebs-Ringer solution for 4 hours sperm penetration began within 1 hour. There is some evidence that this is associated with the completion of sperm capacitation and the acrosome reaction. The results suggest that zona-free hamster ova can be substituted for human ova in the preliminary assessment of the fertilizing capacity of human spermatozoa.
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