Abstract
1. A preliminary report of a new technique as a screening method in the detection of uterine cancer is described whereby the cytology of vaginal smears is studied microscopically by staining with fluorescent dyes (fluorochromes) and using filtered ultraviolet light as a source of illumination.2. The simple and inexpensive apparatus and technique for fluorescence microscopy and fluorescence photomicrography are outlined.3. Directions for the preparation and staining of vaginal smears are given.4. Brief descriptions of the various distinguishing fluorescent colors associated with normal epithelial cells, leucocytes, erythrocytes, Trichomonas vaginalis, moniliasis, and bacteria of the vaginal flora are mentioned.5. The use of this technique for differentiating normal from malignant cells by morphology, degree of fluorescent brilliance, and variation of nuclear and cytoplasmic fluorescent colors is cited.
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