The Use of Tungsten in Potentiometry to Detect Pospat Baffer and Urease Enzyme

Victor H Sihombing,Abd Hakim S

doi:10.29303/jppipa.v7i3.699

Abstract

This study aims to characterize phosphate buffer and urease enzymes through the absorbance spectrum of UV-Vis and FTIR using tungsten as the indicator electrode. The method used in this research is the biosensor potentiometric method carried out in the Laboratory of the Faculty of Mathematics and Natural Sciences, State University of Medan and the Beacukai Belawan Medan laboratory. The absorbance characterization of electrolyte solutions in various compositions using UV-Vis showed that phosphate buffer solution 0.001 M pH 7.5+KCl 0.001 M + urea 0.001 M+3 drops urease enzyme had the highest absorbance compared to electrolyte solutions with phosphate buffer and urea content. Likewise, the FTIR results showed the same thing where phosphate buffer solution was 0.001 M pH 7.5 + KCl 0.001 M + urea 0.001 M + 3 drops urease enzyme had the highest% T (transmission) pattern of phosphate buffer solution and urea. The urease enzyme in this study functions as a catalyst. Based on UV-Vis and FTIR characterization, it was concluded that the phosphate buffer solution of 0.001 M pH 7.5+KCl 0.001 M + urea 0.001 M + 3 drops of urease enzyme was the best.

Highlights

A potentiometric sensor is a device that measures the voltage between two indicator electrodes and a reference electrode depending on the concentration of the analyte, without contradicting the electrochemical cell (Khopkar, 1990; Skoog, 2007; Wang et al, 2008)
Characterization of UV-Vis In Figure 1 (a) it can be seen that an absorbance peak has not been formed at a wavelength of 200 - 300 nm, (b) an absorbance peak is formed at a wavelength of 240 nm with an absorbance peak height of 5.6939 and (c) an absorbance peak is formed at a wavelength of 257 nm with peak height absorbance of 9.7281 with tracking at a wavelength between 200 - 400 nm
In the first absorption area of the solution (b) phosphate buffer 0.001 M pH 5.5 + KCl 0.001 M + urea 0.001 M + 3 drops urease enzyme is at wave number 3435.27 with a transmission of 2.63 and these results indicate the presence of N - H groups at wave number 2066.65 with a transmission of 86.47 and these results indicate the presence of the O-H group

Summary

Introduction

A potentiometric sensor is a device that measures the voltage between two indicator electrodes and a reference electrode depending on the concentration of the analyte, without contradicting the electrochemical cell (Khopkar, 1990; Skoog, 2007; Wang et al, 2008).The analyte binds the bioreceptors to the surface of the indicator electrode in a buffer solution, resulting in a potential difference between the two electrodes. A potentiometric sensor is a device that measures the voltage between two indicator electrodes and a reference electrode depending on the concentration of the analyte, without contradicting the electrochemical cell (Khopkar, 1990; Skoog, 2007; Wang et al, 2008). Potentiometric cells require an electrolyte solution consisting of a buffer as a voltage stabilizer, the urease enzyme functions as a catalyst (accelerator of the enzymatic reaction process), KCl to see the activity and sensitivity of the solution and urea as an analyte with a certain composition in order to obtain optimum results (Gosser, 1993; Hakim, 2018). Potentiometric cell ISE (Ion Selective Electrode) electrolyte solution consisting of phosphate buffer, KCl, urease and urea enzymes.

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Conclusion