Abstract
Abstract : We have constructed vectors for the high-level expression of the anthrax toxin genes. We have cloned the T7 RNA polymerase gene downstream from the IPTG-inducible promoter from pSI-1. IPTG induces expression of the T7 RNA polymerase when the 1acI repressor is inactivated. This integration plasmid has been inserted into the genome of Bacillus anthracis. Shuttle vectors for the expression of the individual anthrax toxin genes (derived from pDRl8l) consists of the replication components from pUBi110, a kanamycin resistance gene and the multi cloning sequence from pET21a, which contains the T7 RNA polymerase promoter and terminator. The individual toxin genes have been inerted into this plasmid. Six recombinant IZAP clones which contain B. anthracis DNA sequences homologous to the spoOH gene of B. subtilts have been isolated. The spo0H gene in the Bacillus species is required for sporulation This gene will be used to produce an asporogenic B. anthracis.
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