Abstract
SummaryA simple technique for differentiation of herpes simplex virus type 1 from type 2, using growth variations in tissue culture and temperature sensitivity is described. All HSV-2 isolates were capable of producing distinct microplaques in chick embryo cells while the newly isolated HSV-1 strains failed to do so. Newly isolated HSV-1 strains showed similar or slightly lower titers in rabbit kidney when incubated at 35 or 40°. In contrast, the HSV-2 isolates generally showed distinctly lower titers or delayed CPE at 40°. Virus typings by selective growth in cell culture and temperature markers were confirmed by neutralization and/or immunofluorescence tests. Since both types of HSV can be isolated from any site of the body, the availability of a simple laboratory technique for distinguishing between the two provides physicians and diagnostic virology laboratories with a means for rapid and accurate diagnosis of herpetic infections.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
