Abstract
Sucrose density gradients were used to isolate both free virus from midgut cells of the forest tent caterpillar, malacosoma disstria, infected with a cytoplasmic-polyhedrosis virus, and virus released when polyhedra were dissolved in weak alkali. Electron micrographs showed a high degree of purity of the virus preparations, and ultraviolet absorption spectra demonstrated typical nucleoproteins.
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