Abstract
Embryogenic cultures of lucerne (Medicago sativa L.) cv. Robot have been established and propagated on medium containing yeast extract. These cultures consisted of unorganized callus tissue bearing embryogenic centres which increased in size during subculture, yielding new regenerated somatic embryos at the end of each 20-d subculture. A development in the propagation of the embryogenic cultures was the establishment of single embryo culture in hormone-free medium where, in selected cases, the process of recurrent somatic embryogenesis (RSE) took place on the hypocotyl of explanted embryos. The process was independent of supporting callus tissue and occurred on simple defined medium. Single embryos underwent either plantlet development or continued RSE on the hypocotyl. One third of the regenerated plantlets showed RSE after the two to three trifoliate leaf stage. In these cases shoot development stopped and only somatic embryo production took place. In vitro cloning of regenerated plantlets allowed us to reproduce each particular genotype before transplantation into soil.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.