The Use of Single Phrenic Axon Recordings to Assess Diaphragm Recovery after Cervical Spinal Cord Injury

Abstract

Electrophysiological recordings taken from the whole phrenic nerve have been utilized previously to describe the gradual increase in functional recovery of a hemidiaphragm paralyzed by ipsilateral C2 hemisection during the crossed phrenic phenomenon (CPP). Although the increase in activity has been temporally correlated with hemisection-induced morphological alterations of the phrenic nucleus, suggesting an association of the increased activity with the morphological alterations, whole phrenic nerve recordings during the CPP can provide only limited information. The purpose of the present study, therefore, was to use phrenic single-axon recording techniques to better understand the mechanisms underlying the recovery of respiratory activity during the expression of the CPP. Recordings from the whole phrenic nerve on the right side and from small fascicles of the phrenic nerve that show only the activity of single phrenic axons (units) on the left side were made in the neck before left spinal hemisection and during the CPP. The results indicated that there were two types of units firing before and during the CPP: an early- and a late-firing unit based on the time of their firing onset in relation to whole phrenic nerve activity. Ten early units and 25 late units were identified according to the shape of their spikes before hemisection as well as during the CPP. In addition to these units, 20 new units were recruited during CPP activity. These new units were mainly of the late-onset type. The results also indicated that there was a significant increase in the frequency of firing of both early and late units. The results specifically indicate therefore that the increase in respiratory activity recorded previously in the whole phrenic nerve during the CPP is most likely due to: (i) an increase in firing frequency for both early- and late-firing units and (ii) a recruitment of predominantly late-firing units into the CPP response. These results are important in understanding more completely the mechanisms that can facilitate recovery of the diaphragm after spinal cord injury.