Abstract

One hundred and forty lots of barley seed were subjected to an embryo test for the mycelium of Ustilago nuda (Jens.) Rostr. Ten samples of 100 embryos were examined from each lot. It was found that the use of sequential sampling, instead of single or double sampling, in making routine tests for loose smut in barley seed, would result in a considerable saving of time and effort as far as determining whether seed stocks contain more or less than the percentage of smut set as the limit of tolerance. However, if the growers require a more exact knowledge of the level of infection in their stocks of seed, it would be preferable to examine 1,000 embryos from each stock, using the single sampling method.

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