Abstract
A critical assessment is made of the separation of thyroxine, free and bound to plasma proteins, and of iodide by means of gel filtration on Sephadex. The following sources of error are discussed: (I) Spontaneous degradation of thyroxine to iodide. (2) The effect of the shape and size of the column on the relative proportions of the separated substances. (3) The effect of the adsorption of free thyroxine on the Sephadex material. The ways by which thyroxine is eluted from the column are also discussed and the error for the method is determined.
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