The use of SDS PAGE to reveal the hybridity of wheat (Triticum aestivum) crosses and genetic diversity analysis

Abstract

An attempt was made to test the hybridity of crosses through SDS PAGE analysis. A total of 31 wheat (Triticum aestivum) genotypes were taken for the study out of which 14 genotypes were parental lines (differing in drought tolerance trait) and 17 genotypes were random crosses among the 14 parental lines. Protein bands were scored with their Rf values which ranged from 0.24 to 0.95. A total of 330 bands were obtained. Secondly the genetic diversity was estimated by comparison of the banding pattern in the protein profiles of the genotypes. The bands of Rf values 0.28, 0.32 and 0.60 were present in all the drought tolerant genotypes and were absent in susceptible parents so these could be utilised as markers for drought tolerance trait. Similarity matrix table was made based on the presence and absence of bands. Maximum similarity (0.9041 similarity coefficient) was observed between WH 730 and its cross WH730 X UP 2554 and two crosses WH 730 X UP 2425 & WH 730 X UP 2554. The lowest similarity (0.6575 similarity coefficient) was observed in two cases i.e. between the crosses WH 730 X UP 2338 & HI 385 X UP 2338 and between the crosses HI 385 X UP 2338 & WH730 X UP 2425.