The Use of Reference Materials In the Analysis of Medicinal Plant Raw Materials and Herbal Medicinal Products

Abstract

The need to improve pharmacopoeial approaches to the standardization of medicinal plant raw materials (MPRMs) and herbal medicinal products (HMPs), and the need to ensure the implementation of the principle of «cross-cutting» standardization in the series «medicinal plant raw material – phyto-substance – herbal medicinal product» determine the relevance of developing new, more rational approaches to the analysis of objects of plant origin.The purpose of the research was the theoretical and experimental substantiation of the use of reference materials in the development of assay procedures on the example of certain types of plant raw materials and products based on it from the standpoint of the chemical composition, stability and physicochemical properties of the biologically active compounds contained in them.Assay of active substances was carried out by high performance liquid chromatography (HPLC) using a Milichrome-6 chromatograph with a spectrophotometric detector in the ultraviolet (UV-) region. The UV spectra were recorded using a Specord 40 spectrophotometer. The measurement results were processed using the WinASPECT and Microsoft Excel 2016 programs.As a result of research, procedures for assay of syringin in the bark of Syringa vulgaris, and in the rhizomes and roots of Eleutherococcus senticosus, rosavin and salidroside in the rhizomes and roots of Rhodiola rosea, arbutin in the leaves of Arctostaphylos uva-ursi and Vaccinium vitis-idaea, isosalipurposide in the flowers of Helichrysum arenarium, the amount of anthracene derivatives in fresh leaves of Aloe arborescens were developed and validated. Spectrophotometric methods for determining the amount of biologically active phenylpropanoids in terms of eleutheroside B (syringin) in MPRMs and HMPs of Eleutherococcus senticosus and the amount of aralosides in the roots of Aralia Manchurian were introduced. Based on the data obtained, the scientific rationale for the use of reference materials of syringin (Syringa vulgaris bark, Eleutherococcus senticosus rhizomes and roots), rosavin and salidroside (Rhodiola rosea rhizomes and roots), the sum of ammonium salts of aralosides (Manchurian aralia root), arbutin (Arctostaphylos uva-ursi and Vaccinium vitis-idaea leaves), mixtures of aloins A and B (Aloe arborescens fresh leaves) in the analysis procedures was provided. The concept of a systematic approach to the analysis of medicinal plant raw materials and products based on it was formulated.