Abstract
Fusion proteins in which the N-terminus of a G protein f subunit is attached in frame to the C-terminal tail of a G-protein-coupled receptor have become widely used as experimental systems to explore the quantitative details of ligand stimulation of specific receptor G-protein combinations. In part, this reflects that they function as agonist-activated GTPases that behave with simple Michaelan kinetics. They have also been used to explore the effects of mutation in both receptor and G protein on information transfer, ligand regulation of posttranslational acylation, and the mechanism and potential selectivity of regulators of G-protein signaling.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
