The use of RAPD markers to Detect the genetic diversity of E. coli isolates from Kirkuk city environment

Abstract

The environmental samples we were collected about 130 samples from the soil and water and were dividing to (71) , (59) water and soil samples resectively.
 The growth isolation on the different cultural media was diagnosed from the appearance, microscopically, and cultural characteristics.
 The results of the diagnosis for the environmental samples showed that (51) of the isolations refers to E. coli bacteria about (45.9%) divvied to 33 isolates from the water by (52.4%) and (18) isolates from the soil by (37.5%).
 The ecological isolates was studied against nine antibiotics, broadly the isolates variables wherefrom the resistance of the antibiotic. The highest resistance was to the antibiotic cephalexin which reach to (80.4%).while there was no resistance for antibiotics (Amikacin) and (5.5%) to chloramphenicol
 It has been selected 24 clinical isolations, and 24 ecological isolates from E. coli isolates depending on here resistance to many of antibiotics. To study the genetic variation for these isolates and the genetic markers it has been used a type of molecular markers which is depending on PCR technical it is: Randomly Amplified Polymorphic DNA (RAPD), and polymorphism bands then the observed data were enters to the computers for statistical analysis according to NTSYS-PC program, version 2.02 for this kind of the researches.