Abstract
1. 1. Radioactive choline services as a highly efficient and specific label for the lecithin of rat liver microsomes, provided that it is administered with non-radioactive amino acids to inhibit incorporation of radioactivity into protein. 2. 2. The radioactivity of lecithin can be directly measured by counting acid-precipitated fractions. The extraction of lipids before counting is thus avoided, allowing the rapid monitoring of multiple samples. 3. 3. With a labelling time of 30–35 min choline incorporation by rat liver in vivo occurs selectively into the microsomal fraction. Evidence is presented that this incorporation does not occur by a simple exchange reaction, but probably via the pathway involving phosphorylcholine and CDP-choline.
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