THE USE OF QUERCETIN FOR SPECTROPHOTOMETRIC DETERMINATION OF SOME CNS ACTING DRUGS

Abstract

Two simple and sensitive spectrophotometric methods for the determination of seven CNS acting drugs namely; haloperidol, droperidol, chlorpromazine hydrochloride, thioridazine hydrochloride, imipramine hydrochloride, desipramine hydrochloride and clomipramine hydrochloride are presented. The first method is based on formation of an ion -pair complex between each of studied drugs and oxidized quercetin at pH range from 4.5 to 5.0. The colored complex formed was quantitatively extracted into dichloroethane, filtered over anhydrous sodium sulphate and then measured in the range of528-534 nm. Beer's law was obeyed for all the studied drugs in the concentration range of2-45 µg/ml. The second method is a rapid kinetic method based on in-situ oxidation of quercetin into a red colored compound measured at 515 nm. The rate of reaction between each of studied drugs and oxidized quercetin was followed by measuring the decrease in absorption intensity as a result o f its reduction by each of the studied drugs. The studied compounds were determined in the concentration range of 5- 50 µg/ml using slope, fixed time and variable time methods . The two methods were successfully applied to the analysis of studied drugs in the pure form and in commercial pharmaceuticals. The results were comparable with those obtained with either official or reported methods.