Abstract
Escherichia coli HB101 is a plasmidless, non-fimbriated bacterium. The plasmid pSLM204, which encodes for the production of the adhesive F41 antigen found in enterotoxigenic E. coli, was sub-cloned into E. coli HB101. It is possible to remove the F41 antigen from E. coli HB101(pSLM204) by physical shearing and to monitor its removal by electron microscopy and mannose-resistant haemagglutination with guinea pig erythrocytes. Pyrolysis—mass spectrometry can differentiate between E. coli HB101, HB101(pSLM204) and the latter strain after the removal of F41 fimbriae. This study shows that the difference observed is due largely to F41 antigens on the surface of strain HB101(pSLM204).
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