Abstract
Abstract Changes in the NMR chemical shift of carbon-13 nuclei upon protonation of a nearby basic center are shown to be a useful method of probing the microscopic site of protonation of molecules containing multiple basic centers, such as diethylenetriaminepenta-acetic acid (DTPA). This approach is also shown to support the predominance of the pyridine amine protonated tautomer in the 4-pyridone ↔ 4-hydroxypyridine equilibrium.
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