The use of protein hydrolysate from fish waste as part of microbiological culture media

Abstract

The need to involve in the processing of significant amount of secondary protein-containing raw materials formed during fish cutting, on the one hand, and on the other hand, the shortage of a source of the protein component of microbiological nutrient media determines the relevance of the work. The goal was to study the possibility of using fish protein hydrolyzate obtained by enzymatic hydrolysis from secondary fish raw materials in microbiological nutrient media. Microbiological and physicochemical methods were used to conduct research. When optimizing the developed algorithm for the fermentation process, a mathematical model of the experiment was used with a minimum number of experiments. An algorithm has been developed for obtaining fish protein hydrolyzate from meat and bone waste from cutting pelagic fish (cod). The hydrolysis parameters were optimized: the concentration of the enzyme preparation is 1.33 % of the total mass of waste, the duration of the fermentation process – 3 hours. The characteristics of the resulting fish hydrolyzate are determined: the mass fraction of total nitrogen – 13 %, amine – 3.6 %, water – 4.6 %, sodium chloride – 2.7 %, fat – 0.3 %. An experimental fish hydrolyzate has been studied as a source of protein nitrogen in accordance with the formulation of a microbiological medium used for counting when growing colonies of microorganisms. The effectiveness of the prepared nutrient media was assessed by comparing the performance coefficients of the experimental and control nutrient media, as well as by identifying test microorganisms grown on the experimental medium. It was established that the growth pattern of test cultures both on the studied nutrient medium and on the control medium was almost the same. Test microorganisms retained their biochemical, morphological and cultural characteristics. The research results showed the possibility of using fish protein hydrolyzate obtained by enzymatic hydrolysis from secondary fish raw materials in microbiological nutrient media.