The use of porcine liver (2 → 3)-α-sialyltransferase in the large-scale synthesis of α-Neu p5Ac-(2 → 3)-β- d-Gal p-(1 → 3)- d-Glc pNAc, the epitope of the tumor-associated carbohydrate antigen CA 50

Abstract

α-Neu p5Ac-(2 » 3)-β- d-Gal p(1 » 3)- d-Glc pNAc ( 2) and, α-Neu p5Ac-(2 » 3)-β- d-Gal p-(1 » 3)-β- d- Glc pNAcOMBn were prepared on a large scale by the action of β- d-Gal pNAC (2 » 3)α-sialyltransferase (partially purified from porcine liver) on β- d-Gal p-(1 » 3)- d-Glc pNAc and β- dGal p (1 » 3)-β- d-Glc pNAcOMBn, respectively. The trisaccharide 2 is the epitope of the tumor-associated carbohydrate antigen CA 50, highly expressed in human pancreatic adenocarcinoma.