Abstract
The use of polyazolidinammonium modified with iodine hydrate ions (PAAG) as an adjuvant made it possible to obtain rabbit hyperimmune blood serums for dimethyl-sulfoxide antigen (DA) of a pseudotuberculosis microbe with generic specificity. Antibody titers in ELISA with Y. pseudotuberculosis and Y. enterocolitica cells amounted to 1:25600-1:12800, and with cells of other genera of intestinal microflora 1:100-1:400. The optimal immunizing dose for obtaining hyperimmune yersiniosis serum was a dose of 2 mg DA of Y. pseudotuberculosis per rabbit. Such a dose made it possible to obtain hyperimmune sera with a high titer of specific antibodies with a small consumption of antigen. The optimal concentration of PAAG solution for hyperimmunization of Y. pseudotuberculosis DA rabbits was 1%.
Highlights
Intestinal yersiniosis is registered in many countries of the world and occurs in pigs with a large livestock population
The rabbits of the experimental groups were mixed with the antigen 1:1 with 1% solution of PAAG (DA + PAAG), and the rabbits of the control groups were mixed with physiological saline (DA + PS)
The obtained blood serum was examined by ELISA in reaction with dimethyl-sulfoxide antigen (DA) Y. pseudotuberculosis (20 μg / ml) (Table 1)
Summary
Intestinal yersiniosis is registered in many countries of the world and occurs in pigs with a large livestock population. The circulation of Yersinia enterocolitica (Y. enterocolitica) is detected in other domesticated animals and birds. Pigs are the main source of Y. enterocolitica for human infection [1,2,3,4,5,6,7]. There is less information on the circulation of the pseudotuberculosis microbe in animals than on the circulation of the causative agent of intestinal yersiniosis. Pseudotuberculosis in animals occurs sporadically or in the form of small outbreaks. Infection of people with Yersinia pseudotuberculosis (Y. pseudotuberculosis) occurs through an alimentary route mainly through plant products and the role of animals in human infection is not clear [1,2,3,4]
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