Abstract
We have studied the effects of the antibiotic trichothecin (Trichothecium roseunm) and the systemic fungicide triadimefon on the parasitic activity of Puccinia graminis and on metabolic processes of plants. Trichothecin is an antifimgial antibiotic formed by Trichothecium roseum. This antibiotic is a ketone with empirical formula C19H24O5. Triadimefon is a systemic fungicide (3,3-dimethil-1/1H-1,2,4,-trizolil/4-chlorophenoxi/buthanol-2). The experiments were performed on isogenic lines of wheat Triticum aestivum L. cv. Markiz with different resistance to stem rust. Two strains of wheat were studied: the resistant strain with gene Sr Tt and susceptible strain with gene Sr 9B. Physiological and biochemical tests were performed on the first leaves of wheat seedlings of uninfected and infected plants. The concentrations of trichothecin and triadimefon were selected on the basis of data of inhibitory effect on activity of P.graminis uredospores germinating in moist camera on the surface of 1% water. The solution concentration of trichothecin and triadimefon which strongly suppressed the uredospores germinating on water agar were chosen for the experiments with plants. The influence of the preparation on the degree of defeat was controlled by the number of pustules on leaves, which appeared om 8-10-th day after the invasion. The number of pustules was counted on 20–30 plants.
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