Abstract
A method is described for ultrastructural study of tissue antigens reacting with serum antibodies by an indirect method using horseradish peroxidase conjugated antiglobulin. The method was applied to slide mounted cryostat sections of fixed tissue. This allowed short incubation times and screening by light microscopy. After short fixation of tissues in 1% formaldehyde, staining at light microscope level was similar to that on unfixed tissue. Increased fixation reduced the staining. The degree of fixation permissible without serious loss of staining varied directly with the titre of the serum antibody. Despite loss of ultrastructural detail, especially with tissues fixed for short periods, the method gave information about localization of antigen not obtainable by light microscopy.
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