Abstract
pAL5000 based vectors, conventionally used to transform Mycobacterium species were used in this study to transform Rhodococcus and Gordonia species. Adequate transformation efficiencies were obtained ranging between 4.5x10 3 – 2.8x10 6 . Additionally, a pAL5000 based vector with suicide function was generated. This vector was transformed into
Highlights
In this study we focused on finding an efficient vector transformation system for Rhodococcus sp. and Gordonia sp. strains which other studies have shown to be biotechnologically relevant
The host range of vectors pNV18,pNV19,pOLYG,pCY104 and pK4 were tested in the bacterial species; Rhodococcus, Mycobacteria and Gordonia utilizing electroporation (Table3)
Forty potential transformants which grew on the antibiotic plates were selected and regrown in the appropriate antibiotic after which the vector was extracted and retransf ormed into E. coli
Summary
They are capable of the biosynthesis of an array of substrates and decomposition and utilization of harmful compounds. Their capacity to degrade recalcitrant compounds can be demonstrated by numerous studies. R. rhodochrous strains have been implicated in the degradation of crude oil, herbicides and pesticides (Harada et al, 2006; Sorkhoh et al, 1989). Cloning vectors have undoubtedly played a tremendous role in understanding prokaryotic genetics. They make possible the determination of gene function and the expression of gene products while contributing valuable information pertaining to metabolic pathways
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