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Abstract
The Ościk’s equation based on the theory of conformal solutions was used to describe the chromatographic process in RP-HPLC. Theoretically calculated values of the logarithms of retention factors (logk) were compared with experimental values for a group of aromatic substances, i.e. alkylbenzenes, PAHs, and polar benzene derivatives. Studies were conducted using two columns: RP-18 and RP-4, as well as water-methanol and water-acetonitrile mixtures as mobile phases.The applicability of Everett’s equation to describe the composition of surface phase was also verified. For this purpose, the excess methanol adsorption isotherm on LiChrospher RP-18e, was experimentally determined.
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