Abstract
o-Nitrophenyl β- d-galactoside was found to serve as a donor of galactosyl units in enzymic transgalactosylation to d-galactose and d-arabinose. The oligosaccharides produced in the reactions were isolated by chromatographic procedures, and structural information on the compounds was obtained. Two oligosaccharides were characterized as 3-β- d-galactopyranosyl- d-arabinose and 6-β- d-galactopyranosyl- d-galactose. Tentative structures, 5-β- d-galactopyranosyl- d-arabinofuranose and 3-β- d-galactopyranosyl- d-galactose, were assigned to two other oligosaccharides. A new chromatographic procedure termed column-paper chromatography was found to be effective for separating compounds with small differences in R f values.
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