Abstract
Abstract— The binding of neutral red to purple membrane has been studied. The intrinsic pKa and the apparent pKa, of bound neutral red were determined by titration and by measuring the binding ratio of neutral red to purple membrane as a function of pH. The surface potential of purple membrane was inferred from the difference between these two pKas. The H+/M412 ratio at different ionic strengths was also measured and compared with the surface potential. The results show that the H+/M412 decreased as the surface potential increased due to decreased salt concentrations. However, this correlation holds only for KCl concentrations higher than 30 mM. At lower salt concentrations, the change in surface potential is always less than the variation in the H+/M412 ratio.
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