Abstract
Plant diseases are one of the most studied subjects in the field of plant science due to their impact on crop yield and food security. Our increased understanding of plant–pathogen interactions was mainly driven by the development of new techniques that facilitated analyses on a subcellular and molecular level. The development of labeling technologies, which allowed the visualization and localization of cellular structures and proteins in live cell imaging, promoted the use of fluorescence and laser-scanning microscopy in the field of plant–pathogen interactions. Recent advances in new microscopic technologies opened their application in plant science and in the investigation of plant diseases. In this regard, in planta Förster/Fluorescence resonance energy transfer has demonstrated to facilitate the measurement of protein–protein interactions within the living tissue, supporting the analysis of regulatory pathways involved in plant immunity and putative host–pathogen interactions on a nanoscale level. Localization microscopy, an emerging, non-invasive microscopic technology, will allow investigations with a nanoscale resolution leading to new possibilities in the understanding of molecular processes.
Highlights
The plant cell wall and its outer cuticle represent the first line of defense to biotic and abiotic stress
In planta Förster/Fluorescence resonance energy transfer has demonstrated to facilitate the measurement of protein–protein interactions within the living tissue, supporting the analysis of regulatory pathways involved in plant immunity and putative host–pathogen interactions on a nanoscale level
We describe the role that Förster/Fluorescence resonance energy transfer (FRET) and localization microscopy has played and probably is going to play in investigating plant– pathogen interaction by highlighting processes occurring at the plant cell wall and being part of cell wall integrity mechanisms
Summary
The plant cell wall and its outer cuticle represent the first line of defense to biotic and abiotic stress. The development of labeling technologies, which allowed the visualization and localization of cellular structures and proteins in live cell imaging, promoted the use of fluorescence and laser-scanning microscopy in the field of plant–pathogen interactions.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have