Abstract
This paper reviews the results of studies of interspecies hybridization, polyploidization, as well as phylogenetic relationships ofSolanumspecies and members of closely related taxa by such molecular cytogenetic techniques as genomic (GISH) and fluorescent (FISH) DNA-DNAin situhybridization. The latter was used to determine the genomic composition and origin of wild species of thePetotasection, while the FISH technique was used for detecting intergenomic collinearity. The combination of these two types of research made possible a comparative analysis of karyotypes and genomes, thus allowing a better understanding of the meiotic interchromosomal interactions in hybrids. This review primarily focuses on the studies of wild allopolyploid potato species and artificially created intergeneric and interspecific hybrids of the genusSolanumand their offspring.
Highlights
Данный обзор посвящен рассмотрению результатов изучения межвидовой гибридизации, полиплоидии и филогенетических отношений видов рода Solanum и представителей близких к нему таксонов, с использованием методов молекулярной цитогенетики, таких как гибридизация ДНК-ДНК in situ: геномная – genomic in situ hybridization (GISH) и флуоресцентная – FISH
This paper reviews the results of studies of interspecies hybridization, polyploidization, as well as phylogenetic relationships of Solanum species and members of closely related taxa by such molecular cytogenetic techniques as genomic (GISH) and fluorescent (FISH) DNA-DNA in situ hybridization
The latter was used to determine the genomic composition and origin of wild species of the Petota section, while the FISH technique was used for detecting intergenomic collinearity
Summary
Данный обзор посвящен рассмотрению результатов изучения межвидовой гибридизации, полиплоидии и филогенетических отношений видов рода Solanum и представителей близких к нему таксонов, с использованием методов молекулярной цитогенетики, таких как гибридизация ДНК-ДНК in situ: геномная – GISH и флуоресцентная – FISH. Отобранные BAC-маркеры, и рекомбинации хромосом родительских видов, а так- вследствие гибридизации фрагмента ДНК вставки с хроже выявлять межгеномные перестройки у исследуемых мосомной ДНК, были локализованы на разных плечах гибридов и форм с интрогрессией генетического материала (Garriga-Calderé et al, 1997; 1998; 1999; Zwierzykowski et al, 2008; Yao et al, 2010; Jeridi et al, 2011; Silva, Souza, 2013; Gavrilenko et al, 2014).
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