The use of laser radiation to assess the bowel viability in an experiment

Abstract

Aims: To investigate the possibility of using laser rays scattering indicators to assess the bowel viability. Methodology: 30 white rats. A loop of the middle part of the small bowel (SB) with the mesentery was ligated in 10 rats. A loop of the middle part of the large bowel (LB) with the mesentery was ligated in 10 rats. The width of the laser rays at wavelengths λ=0.63 and λ=0.4 μm scattering zone (WLRSZ) was measured (in millimeters) on the afferent (AP), efferent (EP) and ligated (LP) parts of the bowel’s walls in the in 6 hours. Sections of bowels were taken for histological examination. The data obtained by measurements in 10 healthy animals were the control. Results: In AP revealed morphological disorders without signs of necrosis, in EP revealed minor changes, in LP revealed necrosis. We used the ratio of the WLRSZ at wavelengths λ=0.63/λ=0.4 μm. Control ratio on SB and LB didn’t differ significantly. AP ratio on SB and LB was not significantly different, but was significantly different from the control. EP ratio on SB and LB was not significantly different and did not differ significantly from the control, but differed significantly from the indicators on the AP. LP ratio indicators on SB and LB was not significantly different, but significantly different from the control, indicators on AP and EP. Conclusion: 1. The ratio of the laser rays at the wavelengths λ=0.63/λ=0.4 μm scattering zone width is statistically significantly different in the bowel parts without deviated viability, with morphological deviations without signs of necrosis, and with necrosis. 2. Ratio indicators > 1.58 units indicate preserved viability, indicators in the range of 1.4-1.35 units indicate morphological deviations without necrosis, indicators < 1.2 units indicate bowel necrosis. 3. The determination of this ratio should be used in patients to assess its informativeness. Aims: To investigate the possibility of using laser rays scattering indicators to assess the bowel viability. Methodology: 30 white rats. A loop of the middle part of the small bowel (SB) with the mesentery was ligated in 10 rats. A loop of the middle part of the large bowel (LB) with the mesentery was ligated in 10 rats. The width of the laser rays at wavelengths λ=0.63 and λ=0.4 μm scattering zone (WLRSZ) was measured (in millimeters) on the afferent (AP), efferent (EP) and ligated (LP) parts of the bowel’s walls in the in 6 hours. Sections of bowels were taken for histological examination. The data obtained by measurements in 10 healthy animals were the control. Results: In AP revealed morphological disorders without signs of necrosis, in EP revealed minor changes, in LP revealed necrosis. We used the ratio of the WLRSZ at wavelengths λ=0.63/λ=0.4 μm. Control ratio on SB and LB didn’t differ significantly. AP ratio on SB and LB was not significantly different, but was significantly different from the control. EP ratio on SB and LB was not significantly different and did not differ significantly from the control, but differed significantly from the indicators on the AP. LP ratio indicators on SB and LB was not significantly different, but significantly different from the control, indicators on AP and EP. Conclusion: 1. The ratio of the laser rays at the wavelengths λ=0.63/λ=0.4 μm scattering zone width is statistically significantly different in the bowel parts without deviated viability, with morphological deviations without signs of necrosis, and with necrosis. 2. Ratio indicators > 1.58 units indicate preserved viability, indicators in the range of 1.4-1.35 units indicate morphological deviations without necrosis, indicators < 1.2 units indicate bowel necrosis. 3. The determination of this ratio should be used in patients to assess its informativeness.