Abstract
We have described a technique for using labelled cations to measure the induction of electrolyte leakage in victorin-sensitive oat root cells caused by the host-specific toxin, victorin. The technique provides a rapid and sensitive way to measure leakage of specific labelled ions. By using the technique, we have demonstrated that the 86Rb leakage follows the same pattern as electrolyte leakage, in general, after victorin treatments. CaCl2 at 50 mM concentrations inhibits victorin activity as measured by 86Rb leakage. 45Ca retention is not affected by victorin the same as 86Rb or electrolytes in general. Some possible implications of the differential effects of victorin on45Ca and 86Rb in relation to the calcium's role in membrane function are discussed.
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