Abstract
Isoperibol solution calorimetry was used to determine in vitro erythrocyte glucose uptake changes as a function of erythrocyte age. Glucose concentrations were determined by phosphorylation with Mg 2+ /ATP 2- in the presence of hexokinase. The differential heat of the standard reaction was found to be -70.3 kJ mol -1 (-16.8 kcal mol -1 ). Lower amounts of glucose were taken up by 'expired,' older than 90 days, cells (0.37 mmol/5 mL packed cells) than by 'fresh,' immediately after blood collection, cells (0.87 mmol/5 mL packed cells). Statistical analyses of the data revealed significant differences in extent of glucose uptake between fresh cells and expired cells (p<0.05).
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