The use of isoimmune sera for identification of hemopoietic cells from blood-injected radiation chimeras.

Abstract

Use has been made of the antigenic differences among mice with respect to both erythrocytes and nucleated cells to identify hemopoietic cells of radiation chimeras. By employing isoimmune sera in agglutination and cytotoxic techniques, it has been shown that long-term leukemoid blood chimeras contain lymphocytes, erythrocytes, and nucleated bone marrow cells of the blood-donor type; blood from normal mice can repopulate lymphoid tissues of radiationbone marrow chimeras, and the blood-derived cells might take part in graft-versus-host immune reactions; and normal peripheral leukocytes from F/sub 1/ hybrid donors can reestablish lymphopoiesis, granulopoiesis, and erythropoiesis when injected into lethally irradiated parent strain mice. Cytotoxic typing of the nucleated hemopoietic cells shows that they are of donor origin. (auth)