The use of iPBS markers to study the genetic diversity of known stock grape varieties

Abstract

The expansion of viticulture in the world is accompanied by the spread of diseases and plant pests. Phylloxera is one of those organisms causing enormous damage to the world’s viticulture. The discovery of the possibility of grafting varieties on stock has led to the development of the breeding of stock originating from North America. Currently, the active use of different types of molecular markers for the identification and study of the genetic diversity of grapes allows to more accurately and efficiently study the biological peculiarities and genetics of plants. Obtaining good reproducibility of the analysis results and revealing the polymorphism between varieties and clones when using different types of markers, you can further use this knowledge for breeding in the selection of crossed pairs. The aim of the study in the present article was to study the common stock grape varieties - hybrids of American species. The article presents a study of the five most famous stock grape varieties: Kober 5BB and 420-A, Paulsen 1103, 101-14 and Richter 57 to identify genetic relationships and polymorphism between samples, based on the use of IRAP and iPBS primers. We used thirteen selected DNA markers that generated a total of 308 polymorphic DNA bands with 54.95 % polymorphism. The effectiveness of iPBS markers was comparable or even more effective than the markers based on retrotransposons. As a result of research, differences and commonality between stocks were clearly demonstrated by both cluster analysis and PCoA analysis.