Abstract
In 2010, the Directive on undesirable substances in animal feed entered into force and for the first time was laid down a maximum limit for nitrite content in sugar industry feed materials such as molasses and beet pulp. Due to a lack of suitable analytical methods for nitrite determination, this study was developed with the aim to standardize a nitrite analytical method in by-products from sugar industry.In this study high performance anion exchange chromatography with conductometric detection was used for determining nitrite and nitrate content of sugar by-products included in feed material.The study confirms the usefulness of the applied ion chromatographic method for the evaluation of the content of nitrite and nitrate in sugar by-products.The results showed that in many samples of beet pulp and molasses the content of “undesirable substances for animal feed” was below 15mgkg−1 (expressed as sodium nitrate at 12% of moisture).
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