The use of ion exchange resins for separation of basic amino acids

Abstract

AbstractThe hexone bases can be separated from each other by adsorption on a column of the cation exchange resin, Zeo‐Karb 215, and elution with bases and acids of suitable strengths. Zeo‐Karb 215 also adsorbs some of the non‐basic amino acids from protein hydrolysates, and these tend to interfere with the elution of the hexone bases. Zeo‐Karb 216 adsorbs hexone bases only from a mixture of the amino acids, but has a lower capacity and is less satisfactory than Zeo‐Karb 215 for separating the bases. The considerable quantities of eluent required for good partitions make the technique unsuitable for the large‐scale production of individual amino acids from hydrolysed proteins. Elution of adsorbed acids by shaking the resin mechanically with eluates required even greater volumes than the column procedure.