Abstract
Hydrogen sulfide is a toxic gas involved in the regulation of some essential biological processes. A novel, precise, accurate and rapid method based on high-performance liquid chromatography with diode array detection for the determination of sulfide ions in human urine sample is proposed. The method involves the derivatization of sulfide with pyrylium salts – (2,4,6-triphenylpyrylium hydrogensulfate(VI) (L1) and 4-[p-(N,N-dimethylamino)phenyl]-2,6-diphenylpyrylium chlorate(VII) (LN1). The separation occurs on InfinityLab Poroshell 120 EC C18 column using acetonitrile and phosphate buffer as a mobile phase. The detectors utilized a wavelength of 371 or 580 nm. The calibration curves were linear in the range of 2–150 μmol L−1 and 1–50 μmol L−1 for L1 and LN1 derivatives, respectively. The samples were found to be stable from sample collection to final analysis. The method was successfully applied to samples from apparently healthy volunteers.
Highlights
Hydrogen sulfide is an endogenous gasotransmitter in the cardio vascular system which plays a similar role to nitric(II) oxide and carbon (II) oxide [1,2]
To choose the optimal value for each of the aforementioned parameters, particular attention was paid to the shapes of the peaks, their surface areas, retention times, and to the complete separation of the derivatives tested from the excess of deri vatizing reagent
The results showed that different concentration of phosphate buffer did not have a significant impact the separation of L3 or LN3 from an excess of derivatization reagents
Summary
Hydrogen sulfide is an endogenous gasotransmitter in the cardio vascular system which plays a similar role to nitric(II) oxide and carbon (II) oxide [1,2]. Hydrogen sulfide is synthesized in various mammalian tissues such as in the brain, cardiovascular system, liver, and kidney [3] by the desulfurization of L-cysteine [4] or L-methionine [5] While it is widely distributed in a variety of organs, hydrogen sulfide plays in teresting roles in the kidneys [6,7]. It participates in the control of renal function and increases urinary sodium. Deficiency of it has been implicated in diabetic kidney injury and associated with hypertension, diabetic nephropathy and hyperhomocysteinaemia [8]
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