Abstract
The peak of the unspecified compound of quantitative analysis of organic acids in biological materials by using high-performance liquid chromatography was identified. This peak corresponded to polygalacturonic acid. A High-performance liquid chromatographic method was developed to identify and quantify of water soluble pectin fraction and insoluble pectin fraction, galacturonic acid and polygalacturonic acid. The method based on the extraction of free water soluble fraction with phosphate buffer (pH 4.8) or with 0.01 N phosphoric acid and on the acid hydrolysis of insoluble pectin fraction in presence 0.1 N HNO3. The pectin fractions were separated and determined on a C610H column using elution with 0.01 N phosphoric acids and ultraviolet detection at 210 nm. Galacturonic acid and polygalacturonic acid were used as internal standard. The calibration curves were presented for each of the standards. The determinations were performed in the linear range of 0.04÷0.17% w/v for both pectin fractions. Proposed procedure can considered as a rapid method for quantitative pectin determination in the food, food nutrition’s food, and pharmaceutical agents.
Highlights
Pectin and polygalacturonic acid have applications as therapeutic and diagnostic pharmaceutical agents such as the magnetic resonance imaging agent Lumen Hance (Gregory, W et al, 1999)
In the other part of this work we report the analysis of water soluble pectin fraction and insoluble pectin fraction in dietary fiber samples by high-performance liquid chromatography method (HPLC) coupled with UV detectors in order to characterise their structure
This paper investigates the application of this HPLC method that can be used to analyze either galacturonic acid, polygalacturonic acid, pectin fractions in dietary fibers or in natural products, pharmaceutical agents
Summary
Pectin and polygalacturonic acid have applications as therapeutic and diagnostic pharmaceutical agents such as the magnetic resonance imaging agent Lumen Hance (Gregory, W et al, 1999). L. et al.,2001; Kerry hoster Caffall et al.,2009) Apart from their hormone-like function in plants, OGAs have recently attracted intense interest since they have been demonstrated to inhibit the adherence of bacteria to epithelial cells and might be used as therapeutic agents In the past years the analysis of water soluble pectin fraction and insoluble pectin fraction in dietary fibers has attracted the interest of the researchers due to their beneficial effects on health. In the other part of this work we report the analysis of water soluble pectin fraction and insoluble pectin fraction in dietary fiber samples by HPLC coupled with UV detectors in order to characterise their structure
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