The Use of Haploid Protoplast Fusion to Combine Cytoplasmic Atrazine Resistance and Cytoplasmic Male Sterility in Brassica Napus

Abstract

This report describes the use of haploid protoplast fusion to create cytoplasmic atrazine resistant (CATR) and cytoplasmic male sterile (CMS) B. napus plants. The study was carried out in parallel with the diploid/diploid protoplast fusion work described by Barsby et al. (1987). Protoplasts from haploids were used because the fusion process reestablishes the diploid condition and precludes the need for nuclear inactivation or cytoplast production in one of the parental protoplast populations. The haploid protoplasts were also well suited for use with a flow cytometer/cell sorter to isolate heterokaryons from the fusion mixtures. The success of this approach was dependent on the development of procedures to isolate haploid lines from CMS and CATR B. napus (Chuong et al., 1987a) and the development of plant regeneration protocols from haploid B. napus protoplasts (Chuong et al., 1987b,c).KeywordsCytoplasmic Male SterileFusion ProductMaintainer LineRestriction Fragment PatternPlant Regeneration ProtocolThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.