The Use of Genetic Modification Techniques in the Fluorescent Visualization of Oxytocin Neurons

Abstract

Oxytocin, a neurohypophyseal hormone, is synthesized in the magnocellular neurosecretory cells located in the paraventricular and supraoptic nuclei of the hypothalamus, and is secreted into the systemic blood flow from the axon terminals. It is well known that plasma oxytocin is involved in contraction of the uterus during parturition and milk ejection reflex during lactation. It has recently come to the attention of researchers that oxytocin receptors are abundant in the brain and oxytocin is involved in higher brain functions such as bonding between parent and child and trust. Since it was difficult to identify neurohypophyseal hormones, oxytocin- and vasopressin-producing neurons in a living cell, we tried to generate transgenic animals that express fluorescent proteins as a tag protein to visualize neurohypophyseal hormones. In this paper we review the use of genetic modification techniques in the fluorescent visualization of oxytocin neurons and its application.