Abstract
An alternative SEM preparative technique is proposed for biological specimens in which cellular diffusible constituents may be retained that are otherwise lost during conventional specimen preparation involving critical point drying. The technique utilizes the copolymerization of glutaraldehyde with carbohy-drazide, and is designated “GACH”. Surface morphology with GACH preparations is as good as, or better than, that preserved using critical point drying. Bulk specimen preparation for elemental analysis has been limited heretofore to frozen-hydrated, freeze drying, freeze substitution, unfixed air dried, and critical point dried specimens. All such techniques offer particular advantages, but with certain limitations. The GACH technique is not being proposed as a superior routine replacement for present biological bulk specimen preparation methods, but rather as a viable alternative technique that allows both the retention of bound ions and acceptable surface morphology without elaborate instrumentation.
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Schedule a callMore From: Proceedings, annual meeting, Electron Microscopy Society of America
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