Abstract
A PCR method was developed employing a single primer (GACA)4 to sex Black vultures (Aegypius monachus), Lappet‐faced vultures (Torgos tracheliotus), and Griffon vultures (Gyps fulvus). Using the (GACA)4 primer several PCR products were generated. One or more PCR products displayed a sex‐specific pattern, i.e. they were only present in females (probably corresponding to repetitive DNA on the W chromosome) but absent in males. The sex ratio of 85 Griffon vultures from Spain was almost 1.
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