Abstract
ELISA was used to detect PVY in crude extracts of virus preparations from artificially infected leaves and tuber sprouts of different potato cultivars, extracts and intact leaf discs of some hosts, as well as some other herbaceous test plants. The ELIS A method was rapid and sensitive enabling PVY detection three days after inoculation of potato seedlings, which was 5–7 days before symptoms appearance. Fifty five days after planting was the best stage for PVY detection in potato sprouts. ELIS A could detect PVY in intact leaf discs without homogenization in solanum, potato, pepper, tomato, datura and wild tobacco plants. It gave positive reaction with extracts containing PVY but not with five other plant viruses.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
