Abstract
DNA extraction and polymerase chain reaction are molecular biology techniques widely used in research, diagnostics and biotechnology industry. In this work, we report a practical course designed for undergraduate students of health, biological sciences and biotechnology areas. The course is five days long and emphasizes basic procedures in practical activities. The students were challenged to use three different protocols for DNA isolation (one using commercial silica column and two, in-house) using no lab animals but themselves. They evaluated the DNA yield, and potential amplification of the extracted DNA by PCR using their own cells genetic material. Students were stimulated to adopt a critical view during the course to develop skills including critical analysis of primary results and possible errors, scientific report writing and a problem solving profile.
Highlights
Biological Sciences and Biotechnology are inherently complex and highly abstract
Professionals of health and biological sciences and biotechnology are challenged with techniques such blood collection and extraction of DNA, evaluation by spectrophotometry and agarose gel electrophoresis as well as polymerase chain
A group of ten undergraduate students of both genders (7 females and 3 males), age 21.4 ± 1.43 (20 to 24 years), 100% single, 100% student occupation, 100% auto-declared white skin color, from São Paulo (10%), Minas Gerais (10%) and Rio de Janeiro (80%) on the fourth period (2nd year) of Biology (n = 6) or Biomedicine (n = 4) courses from the Fluminense Federal University (UFF), Rio de Janeiro—Brazil, were invited to participate in the molecular biology summer course offered at the Molecular Oncology Laboratory of Biology Institute of UFF
Summary
Biological Sciences and Biotechnology are inherently complex and highly abstract. professionals of health and biological sciences and biotechnology are challenged with techniques such blood collection and extraction of DNA, evaluation by spectrophotometry and agarose gel electrophoresis as well as polymerase chainHow to cite this paper: Lázaro-Silva, D. Biological Sciences and Biotechnology are inherently complex and highly abstract. Professionals of health and biological sciences and biotechnology are challenged with techniques such blood collection and extraction of DNA, evaluation by spectrophotometry and agarose gel electrophoresis as well as polymerase chain. How to cite this paper: Lázaro-Silva, D. The Use of DNA Extraction for Molecular Biology and Biotechnology Training: A Practical and Alternative Approach.
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