Abstract
The viability of ram spermatozoa in milk and buffered saline diluents containing dimethyl sulphoxide and in milk diluents alone was investigated during incubation studies at 30 and 5�C. Dimethyl sulphoxide did not contribute to the tonicity of a diluent. Dimethyl sulphoxide (5% v/v) or glycerol in a diluent depressed spermatozoal activity during incubation at 30 or 5�C. Dimethyl sulphoxide retarded motility more than glycerol, especially with incubation at 5�C, whilst glycerol rendered a greater proportion of cells immotile than did dimethyl sulphoxide, this effect being significant only after incubation at 30�C.
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