Abstract
The present study was undertaken to evaluate the effect of a Consciousness Energy Healing (The Trivedi Effect<sup>®</sup>) Treatment based test formulation and medium (DMEM) against skin health parameters using HFF-1, HaCaT, and B16-F10 cells. The test formulation and DMEM were divided into two parts. One part of the test formulation and one part of the DMEM received the Consciousness Energy Healing Treatment by Vaibhav Rajan Parulkar and were defined as the Biofield Energy Treated samples, while the other parts were denoted as the untreated test samples. Cell viability using MTT assay showed more than 75% cells were viable in all the tested concentrations in three cells, indicating that the test formulation was safe and nontoxic. The percent cell proliferation by BrdU assay was significantly increased by 434.14%, 244.77%, and 268.53% in the UT-DMEM + BT-Test Formulation, BT-DMEM + UT-Test Formulation, and BT-DMEM + BT-Test Formulation groups, respectively at 8.75 µg/mL in relation to the UT-DMEM + UT-Test Formulation group. Elastin was significantly (p≤0.001) increased 93.52% and 75.81% in the UT-DMEM + BT-Test Formulation and BT-DMEM + UT-Test Formulation groups, respectively at 10 µg/mL compared to the UT-DMEM + UT-Test Formulation group. Hyaluronic acid was significantly increased by 100.07% (p≤0.05), 41.60%, and 50.10% in the UT-DMEM + BT-Test Formulation, BT-DMEM + UT-Test Formulation, and BT-DMEM + BT-Test Formulation groups, respectively at 0.625 µg/mL in relation to the UT-DMEM + UT-Test Formulation group. Melanin was decreased by 10.09% and 6.51% in the UT-DMEM + BT-Test Formulation and BT-DMEM + UT-Test Formulation groups, respectively at 0.125 µg/mL compared to the UT-DMEM + UT-Test Formulation group. Protection of skin cells after UV-B exposure data displayed that the cell viability was increased by 7.22%, 5.75%, and 8.15% in the BT-DMEM + BT-Test Formulation at 0.625%, 1.25%, and 2.5% µg/mL, respectively compared to the UT-DMEM + UT-Test Formulation group. Wound healing data exhibited significant wound closure and cell migration activities in the HFF-1 cells compared to the UT-DMEM + UT-Test formulation group. Overall, the data suggests that the Biofield Energy Treated DMEM and test formulation demonstrated better skin protection action compared to the untreated DMEM and test formulation. Therefore, the Biofield Energy Treated test herbomineral formulation could be useful for the development of effective cosmetic products for the prevention and treatment of several skin problems such as erythema, contact dermatitis, skin aging, wrinkles and/or change in the skin color, etc.
Highlights
Skin is continuously exposed to pro-oxidant environmental stresses from various sources like air pollutants, ultraviolet (UV) light, chemical oxidants, microorganisms, and ozone
The results showed that the cell viability by MTT assay exhibited more than 75% cells were viable in all the tested concentrations, indicating that the test formulation was safe and nontoxic
The percent cell proliferation data using BrdU assay was significantly increased by 434.14%, 244.77%, and 268.53% in the UT-Dulbecco's modified eagle's medium (DMEM) + Biofield Treated (BT)-Test formulation, BTDMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 8.75 μg/mL compared to the UT-DMEM + UT-Test formulation group
Summary
Skin is continuously exposed to pro-oxidant environmental stresses from various sources like air pollutants, ultraviolet (UV) light, chemical oxidants, microorganisms, and ozone. Zinc is well known to play a critical role in overall human physiology It is an essential cofactor of various metalloenzymes and it protects the skin from UV radiation and has been used for wound healing and to reduce inflammation. Lots of evidence suggest that selenium plays an important role in protecting skin from the harmful effects of UV-B It is an essential trace element is found in many foods including meat, fish, eggs, dairy products, and grains. The extract of Centella asiatica is an effective treatment for small wounds, hypertrophic wounds, burns, psoriasis and scleroderma through promoting the proliferation of fibroblast cells It increases the synthesis of collagen, intracellular fibronectin, and the tensile strength of newly formed skin as well as inhibiting the inflammatory phase of hypertrophic scars and keloids [12]. Based on the excellent outcome of the Biofield Energy Treatment, the authors designed this study to investigate the impact of the Biofield Energy Healing based DMEM and test formulation on various skin health parameters using three cell lines such as human foreskin fibroblast (HFF-1), human keratinocytes (HaCaT), and mouse melanoma (B16-F10) cells
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