Abstract
The objective of this work was to test the effects of three dilutions of semen to glucose–methanol extender ratios 1:3, 1:1 and 3:1 on the sperm motility characteristics of diluted and cryopreserved whitefish spermatozoa. The final concentration of extender compounds was achieved using concentrated extenders. The application of 3:1 dilution ratio allowed for the cryopreservation of up to 3 times more spermatozoa than using the currently recommended method (dilution 1:3). However, changes in sperm motility parameters were observed in response to a lower dilution ratio of semen. Further studies should be carried out to optimize this method regarding particular steps for cryopreservation technologies, especially concerning dilution.
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