Abstract
Abstract : A recursive protocol will be used to create the required Fab fragments. (1) Antibodies with the desired binding functions will be induced with the appropriate immunogen and Fabs obtained from recombinant libraries; (2) Fabs will be screened for antigen binding and those with appropriate affinities isolated, sequenced and overexpressed in E. coli; (3) The structures of selected Fabs will be deduced from modeling and amino acid motifs required for metal ion binding will be introduced by site specific mutagenesis; (4) purified Fabs will be tested for their metal ion affinity and response to a transducing substrate; (5) improvements in the binding and catalytic properties of the Fab will be sought by additional rounds of mutagenesis and/or chain shuffling with the original light or heavy chain libraries.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
