Abstract
Oligonucleotide base-paired duplexes have provided the major source of detailed structural and dynamic information about double helical structure. Polycircular compounds (catenanes) are formed during DNA replication and recombination [1, 2]. Here we report investigations of the possibility of using chemical ligation for the study of feasible types of linear oligonucleotide hybridization on linear and circular templates as well as for the design of polycircular oligonucleotides. Using chemical ligation it has been shown that the way linear oligonucleotide hybridization occurs on circular and linear templates depends on such parameters as: oligonucleotide and template sequences, the length of the duplex formed, and the ligation method. The structure of catenanes is confirmed by Mval restriction endonuclease specific hydrolysis.
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