Abstract
AbstractWe report an in vitro method for assessing ocular irritation by measuring cell lysis of mouse connective tissue (strain L, Clone 929) and rabbit corneal cell lines (SIRC). Lysis of corneal epithelial cells in vivo leads to swelling and subsequent opacification of the underlying corneal stroma. In vitro lysis was determined by the measurement of changes in absorbance (360 nm) of cell suspensions over a 10-min exposure to test materials. These data were expressed as numbers of cells/ml using linear regression. The regression line obtained was linear and similar for both cell types. Loss of viability and spontaneous cell lysis were minimal over the assay interval. In a separate study, rabbit corneal cells were used to assess alteration of cell membrane integrity following 30-min exposures to test materials by measuring trypan blue dye exclusion in these cells. Activities of test materials were ranked according to the lowest concentration capable of producing statistically significant cell lysis as fo...
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